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Reagents. Pipelines. Protocols.











NGS validation of GE events


Validate cutting of multiple

guide RNAs using NGS

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guide rna cloning into u6.3 vector


BsmBI cloning of guide RNAs

into chick U6 vector

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HRMA Assay


Validate cutting of guide RNA(s)

using HRMA

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t7 endonuclease assay



Validate cutting of guide RNA(s)

using T7 endonuclease I

view

TSS LAB | Gene Regulatory Networks in Development and Disease


Weatherall Institute of Molecular Medicine
Radcliffe Department of Medicine
John Radcliffe Hospital, Oxford OX3 9DS, UNITED KINGDOM

+44 (0)1865 222332




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